![4.5 Calculating Transformation Efficiency - Tr a n s fo r m a t i o n Efficiency = #of colonies - Studocu 4.5 Calculating Transformation Efficiency - Tr a n s fo r m a t i o n Efficiency = #of colonies - Studocu](https://d20ohkaloyme4g.cloudfront.net/img/document_thumbnails/edb082ffb5b20503b13802d14b8f551c/thumb_1200_1550.png)
4.5 Calculating Transformation Efficiency - Tr a n s fo r m a t i o n Efficiency = #of colonies - Studocu
![SOLVED: Calculate the DNA concentration and transformation efficiency for below scenario: 50 ng of DNA is ligated in a 20 μL reaction. After ligation, the reaction is diluted 2-fold and 5 μL SOLVED: Calculate the DNA concentration and transformation efficiency for below scenario: 50 ng of DNA is ligated in a 20 μL reaction. After ligation, the reaction is diluted 2-fold and 5 μL](https://cdn.numerade.com/ask_previews/7a483a03-c930-4673-ae06-82b9780eec4d_large.jpg)
SOLVED: Calculate the DNA concentration and transformation efficiency for below scenario: 50 ng of DNA is ligated in a 20 μL reaction. After ligation, the reaction is diluted 2-fold and 5 μL
![SOLVED: 1) Calculate the transformation efficiency using the following data (8 pts): PGLO concentration 0.08 pg VL Plasmid volume 10 pL Final volume in tube 510 pL Volume dispersed in agar 100 SOLVED: 1) Calculate the transformation efficiency using the following data (8 pts): PGLO concentration 0.08 pg VL Plasmid volume 10 pL Final volume in tube 510 pL Volume dispersed in agar 100](https://cdn.numerade.com/ask_images/7c362ff11cce433bb5db910e7e902013.jpg)
SOLVED: 1) Calculate the transformation efficiency using the following data (8 pts): PGLO concentration 0.08 pg VL Plasmid volume 10 pL Final volume in tube 510 pL Volume dispersed in agar 100
![SOLVED: Transformation efficiency is an indication of how well your procedure works Transformation efficiency is the number transformnants (cells that take up the plasmid) in the entire culture per microgram of plasmid SOLVED: Transformation efficiency is an indication of how well your procedure works Transformation efficiency is the number transformnants (cells that take up the plasmid) in the entire culture per microgram of plasmid](https://cdn.numerade.com/ask_previews/038cbda7-247b-43d6-8212-5dff9d2e2177_large.jpg)
SOLVED: Transformation efficiency is an indication of how well your procedure works Transformation efficiency is the number transformnants (cells that take up the plasmid) in the entire culture per microgram of plasmid
![SOLVED: Exercise C: Calculating transformation efficiency Table 1 . Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA DNA Diluted? added? Y or N Volume Concentration Total DNA SOLVED: Exercise C: Calculating transformation efficiency Table 1 . Transformation efficiency of E. coli cells transformed with plasmid pUC19 Plate Plasmid DNA DNA Diluted? added? Y or N Volume Concentration Total DNA](https://cdn.numerade.com/ask_images/0bcd1cd18f044dfc9e70ed60df74dcfb.jpg)